{"id":170,"date":"2018-02-06T12:51:01","date_gmt":"2018-02-06T12:51:01","guid":{"rendered":"https:\/\/more.bham.ac.uk\/tapas\/?page_id=170"},"modified":"2018-02-06T12:51:04","modified_gmt":"2018-02-06T12:51:04","slug":"esr-14-crispr-cas9-based-generation-of-genetic-mouse-models-to-assess-platelet-receptor-targeting","status":"publish","type":"page","link":"https:\/\/more.bham.ac.uk\/tapas\/esr-14-crispr-cas9-based-generation-of-genetic-mouse-models-to-assess-platelet-receptor-targeting\/","title":{"rendered":"ESR 14: CRISPR\/Cas9 based generation of genetic mouse models to assess platelet receptor targeting"},"content":{"rendered":"<p><strong>Host:\u00a0Universitaetsklinikum Wuerzburg, Germany<\/strong><\/p>\n<p><strong>Supervisory team:<\/strong> Bernhard Nieswandt and Attila Braun (University of W\u00fcrzburg, Germany), Johan Heemskerk and Marijke Kuijpers (CARIM, University of Maastricht, The Netherlands)<\/p>\n<p><strong>Project locations:<\/strong> University of W\u00fcrzburg (Year 1 and 2); University of Maastricht (Year 3)<\/p>\n<p><strong>Joint PhD Degree:<\/strong> University of W\u00fcrzburg and University of Maastricht<\/p>\n<p><strong>Project Details:<\/strong> In this project, we will generate novel transgenic mouse models to study mechanisms of platelet receptor (GPVI, CLEC-2) signalling in the settings of thrombosis, haemostasis and thrombo-inflammatory diseases. A CRISPR\/Cas9 based gene editing platform will be established (in W\u00fcrzburg) to test basic mechanisms of early ITAM receptor signalling steps identified within the TAPAS consortium; and to validate targeting strategies to modulate the function of these receptors <em>in vivo<\/em> (W\u00fcrzburg) and <em>in vitro <\/em>(Maastricht). This ambitious project will combine state-of-the-art gene editing technology and in-depth biochemical and cell biological studies on platelet receptor functions from single cell to whole body. This project will closely interact with other projects and will provide an essential translational platform within the TAPAS consortium.<\/p>\n<p><strong>Desirable student skills:<\/strong> Genetics, Cell biology, Pharmacology.<\/p>\n<p><a href=\"https:\/\/more.bham.ac.uk\/tapas\/research\/\">Back to project list<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Host:\u00a0Universitaetsklinikum Wuerzburg, Germany Supervisory team: Bernhard Nieswandt and Attila Braun (University of W\u00fcrzburg, Germany), Johan Heemskerk and Marijke Kuijpers (CARIM, University of Maastricht, The Netherlands) Project locations: University of W\u00fcrzburg (Year 1 and 2); University of Maastricht (Year 3) Joint PhD Degree: University of W\u00fcrzburg and University of Maastricht Project Details: In this project, we&#8230;  <a class=\"excerpt-read-more\" href=\"https:\/\/more.bham.ac.uk\/tapas\/esr-14-crispr-cas9-based-generation-of-genetic-mouse-models-to-assess-platelet-receptor-targeting\/\" title=\"Read ESR 14: CRISPR\/Cas9 based generation of genetic mouse models to assess platelet receptor targeting\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":53,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-170","page","type-page","status-publish","hentry"],"featured_image_urls_v2":{"full":"","thumbnail":"","medium":"","medium_large":"","large":"","1536x1536":"","2048x2048":"","bones-thumb-600":"","bones-thumb-300":"","promobox":"","ab-block-post-grid-landscape":"","ab-block-post-grid-square":"","post-thumbnail":""},"post_excerpt_stackable_v2":"<p>Host:\u00a0Universitaetsklinikum Wuerzburg, Germany Supervisory team: Bernhard Nieswandt and Attila Braun (University of W\u00fcrzburg, Germany), Johan Heemskerk and Marijke Kuijpers (CARIM, University of Maastricht, The Netherlands) Project locations: University of W\u00fcrzburg (Year 1 and 2); University of Maastricht (Year 3) Joint PhD Degree: University of W\u00fcrzburg and University of Maastricht Project Details: In this project, we will generate novel transgenic mouse models to study mechanisms of platelet receptor (GPVI, CLEC-2) signalling in the settings of thrombosis, haemostasis and thrombo-inflammatory diseases. A CRISPR\/Cas9 based gene editing platform will be established (in W\u00fcrzburg) to test basic mechanisms of early ITAM receptor signalling steps&hellip;<\/p>\n","category_list_v2":"","author_info_v2":{"name":"lastcs","url":"https:\/\/more.bham.ac.uk\/tapas\/author\/lastcs\/"},"comments_num_v2":"0 comments","featured_image_src":null,"featured_image_src_square":null,"_links":{"self":[{"href":"https:\/\/more.bham.ac.uk\/tapas\/wp-json\/wp\/v2\/pages\/170","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/more.bham.ac.uk\/tapas\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/more.bham.ac.uk\/tapas\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/more.bham.ac.uk\/tapas\/wp-json\/wp\/v2\/users\/53"}],"replies":[{"embeddable":true,"href":"https:\/\/more.bham.ac.uk\/tapas\/wp-json\/wp\/v2\/comments?post=170"}],"version-history":[{"count":1,"href":"https:\/\/more.bham.ac.uk\/tapas\/wp-json\/wp\/v2\/pages\/170\/revisions"}],"predecessor-version":[{"id":171,"href":"https:\/\/more.bham.ac.uk\/tapas\/wp-json\/wp\/v2\/pages\/170\/revisions\/171"}],"wp:attachment":[{"href":"https:\/\/more.bham.ac.uk\/tapas\/wp-json\/wp\/v2\/media?parent=170"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}